Effect of fertilization application on population and diversity of actinomycetes from rhizosphere soils of Sorghum bicolor

Ade Lia Putri Viewed 86 times, Downloaded 85 times

Abstract


The aim of the study was to observe the effect of gradient-N fertilization, compost, microbial inoculant and its combination on population, diversity of actinomycetes, and their antibacterial and enzymatic activities from rhisosphere soils of Sorghum bicolor. Sorghum were cultivated in different fertilizers treatments. Fertilizer treatments including gradient-N chemical fertilizer alone (0%, 50% or 100% NPK) (TN0, TN50, TN100), gradient-N chemical fertilizer + microbial inoculant (TI0, TI50, TI100), gradient-N chemical fertilizer + compost (CN0, CN50, CN100 ), gradient-N chemical fertilizer + compost + microbial inoculant (CI0, CI50, CI100). Soil samples were collected from each rhizospheres of sorghum that has been treating with fertilization application for 100 days. The samples were air dried at room temperature for 7 days. One gram of each air-dried soil sample was pretreated with 0.05% sodium dodecyl sulfate (SDS). Humic acid vitamin agar (HVA) was used as selective media for isolation of actinomycetes. The application of different fertilizer treatments significant effected of population actinomycetes. The highest population of actinomycetes was isolated from rhizosphere soil with the application of CI50. The lowest population of actinomycetes was isolated from rhizosphere soil with the application of CN100 and CN50. A total 126 isolates of actinomycetes were obtained from 12 samples of rhizosphere soil from various treatment based on morphological characteristics. Among the 126 isolates, 95 isolates had aerial mycelium, and only 24 isolates produced soluble pigment into medium. The diversity of actinomycetes in all fertilizer treatments with addition of 50% NPK (except CI50) was higher than that in the other fertilizer treatments. Among the 126 isolates, 45 isolates showed antibacterial activity against B. subtilis. 54 isolates showed activity against E. coli, 101 isolates showed to produce cellulase, and 44 isolates showed produce phosphatase.

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