Ethanol Production Using Cellulolytic, Xylanolytic and Fermentative Yeast on Cassava Waste

Atit Kanti, I Made Sudiana Viewed 100 times, Downloaded 173 times

Abstract


The objective of study was to produce ethanol using cellulolytic yeast SporidiobolusruineniaeBS-070 and fermentative yeast Saccharomyces cerevisiaeJSAT13-2-Y249 on cassava waste. Cassava waste was selected as substrate for ethanol production since it contained high polysaccharide. The process for ethanol production was divided by two steps. First step was hydrolyses of polysaccharide into fermentable sugar which was performed by SporidiobolusruineniaeBS-070. The second step was fermentation by fermentative strain Saccharomyces cerevisiaeJSAT13-2-Y249. Very high fermentable sugar was achieved after 48 hours incubations, and maximum ethanol (4500 mg/L) was achieved after 96 hours incubation. The co-culture of SporidiobolusruineniaeBS-070 and fermentative yeast Saccharomyces cerevisiaeJSAT13-2-Y249 are quite promising to produce biofuel ethanol on cassava waste. This implies the fermentation of cassava waste will solve environmental problem and produce sustainable biofuel.


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