Screening and Physiological Characterization of Rice Rhizosphere Bacteria from Coastal Soil that Produce Indol Acetic Acid in Saline Condition

Dwi N. Susilowati, Mamik Setyowati Viewed 97 times, Downloaded 124 times

Abstract


Climate change due to global warming increase water sea levels. Increasing the frequency of rising sea levels to the mainland can cause salinity problems for agricultural land in coastal areas. It will affect on plant productivity. Planting varieties of crops tolerant to salinity is one of the solutions to overcome this problem. Application of indigenous Plant Growth Promoting Rhizobacteria (PGPR) are expected to further increase the tolerancy of crops to salinity. This study was conducted to select a number of rhizobacteria isolates from rice field which were tolerant to saline conditions and capable of producing phytohormonesauxin (IAA). A total of 48 rhizobacteria isolates from coastal rice field of Patimban (Ptb) and Eretan (Er), West Java have been analyzed. Among 48 isolates, 12 selected isolates produced IAA>30 ppm. Isolate Er B1.3 and Er B1.7a have ability to grow on minimal salt medium content with 15% NaCl. Both isolates is still capable of producing IAA with consecutive concentrations at 1,55 ppm and 3,1 ppm on minimal medium salt content with 10% NaCl. IaaM gene identification results prove that isolates Er B1.3 and Er B1.7a producing IAA through the IAM pathway. Based on 16S rRNA sequencing results known isolates Er B1.3 and Er B1.7a are closely related to Brevibacterium sp. and Aeromonas sp., respectively.


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