Isolation and Screening for IAA production, Nitrogen Fixation, P-Solubilization and Cellulolytic Activity of Plant Growth-Promoting Rhizobacteria from Imperata cylindrica Grasslands

Dinihari Indah Kusumawati, Sri Widawati, Puspita Lisdiyanti, I Made Sudiana Viewed 197 times, Downloaded 471 times


Indigenous Plant Growth-Promoting Rhizobacteria (PGPR) are naturally occur for enhancement of soil fertility, producing plant growth hormones, nitrogen fixing, bio-composting and biodegradation of organic substances of soil without damaging the ecosystem. The objectives of this study was to isolate beneficial microbes using direct and enrichment methods on specific media, and screen these isolates for PGPR traits of Rhizobium-like strains, Azotobacter-like strains, Azospirillum-like strains and phosphate solubilizer-like strains isolated from four Imperata grasslands in Indonesia. About 208 isolates were isolated using direct and enrichment method from Cibinong (CSC); Central Kalimantan (Katingan) and Nusa Tenggara Timur (SV and RM) on specific media screened for IAA, phosphate solubilization, nitrogen fixation and cellulolytic activity. This study showed that enrichment method was effective for isolation of beneficial microbes. One hundred and seven isolates assayed exhibited ability to produce IAA about 200 µg/ml. One hundred sixty four isolates appeared solubilized of Ca-P complex and release orthophosphate. Ninety nine isolates showed ability to fix nitrogen and 24 isolates were able to degrade cellulose. 

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