Growth and Productivity Parameters of 4 Insertional-Mutant-rice Lines under Salinity Stress and their Insertion Patterns

Vincentia Esti Windiastri, Anky Zannati, Satya Nugroho Viewed 119 times, Downloaded 61 times

Abstract


This experiment was aimed to observe the agronomy traits of 4 insertion-mutant-transgenic-Nipponbare rice lines in salinity stress condition and to evaluate the insertion patterns. The lines, (60, 170, 480 and 654), had been selected for their salinity tolerance from previous salinity test experiments. This experiment was conducted from the germination stage until the harvest time by planting the experiment plants in poly-bags and put them in flow tanks. The rice plants were treated with electrical conductivity of 3 mS/cm by means of NaCl using tap water. Nipponbare rice was used as wild type control. Amplifications for hpt and bar genes were conducted using their specific primers and the agronomy traits were measured of the experiment plants from germination stage, vegetative stage (plant height, tiller number) and reproductive stage (heading, seed number, productive tiller number). The observation of agronomic traits of the 4 mutant rice lines showed that each rice mutant lines of 60, 170, 480 and 654 has their own superior agronomic traits. However, the 170 rice mutant line is dominating the agronomic traits from germination and vegetative stage when treated with salinity stress. It has the smallest reduction percentages of germination, plant height in germination stage and length of flag leaves. In the productive stage, the line with the best reduction percentage for average of seed number per tiller was line 60; while the mutant rice line with the best reduction percentages of tiller number and plant height was line 654. The result of the PCR amplifications of hpt and bar genes showed different insertion patterns of each mutant line.


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