Tri Warseno, Ni Kadek Erosi Undaharta, Ema Hendriyani Viewed 249 times, Downloaded 206 times


Begonia salaziensis Warb. is categorized critically endangered based on the IUCN Red List ver. 2.3. and need to be propagated to conserve. This study aims to investigate the effect of using BA and IAA plant growth regulator type and concentration on the growth and development of B. salaziensis culture. The study was arranged in a Completely Randomized Design (CRD) factorial consisting of 2 factors, i.e. the concentration of BA: 0 mg/l; 1 mg/l; 2 mg/l; and IAA concentration: 0 mg/l; 1 mg/l; 2 mg/l. Each treatment was repeated 10 times. To determine B. salaziensis germination the cultures were observed after 12 weeks. The results showed that the combination of BA with IAA on multiplication media had a significantly different effect on the number of shoot, average shoot height, and average number of shoot. The percentage of seed germination on media MS0 was 85%. The best multiplication of B. salaziensis shoots (9.3 ± 1.15) was obtained in MS media supplemented with 2 mg/l of IAA and 1 mg/l of BA. The lowest shoot multiplication (3.6 ± 0.84) was obtained on the control media (MS0) without addition of plant growth regulators, while the highest average length of shoots (1.87 ± 0.26 cm) was obtained on MS media with the addition 1 mg/l of IAA and BA 2 mg/l of BA. The lowest average length of shoots (0.67 + 0.25 cm) obtained on MS media with the addition of 2 mg/l of BA. The percentage of surviving planlets on acclimatization stage was 88.28%.


Begonia salaziensis, conservation, in vitro, multiplication, plant growth regulators.

Full Text:



Amalia, R., T. Nurhidayati, & S. Nurfadilah. 2013. Pengaruh jenis dan konsentrasi vitamin terhadap pertumbuhan dan perkembangan biji Dendrobium laxiflorum J.J Smith secara in vitro. Jurnal Sains dan Seni Pomits 1(1): 1-6.

Bapat, V.A., G.B. Dixit, & S.R. Yadav. 2008. Rescue of endangered plants through biotechnological applications. National Academy Science Letters 31: 201-210.

Boesewinkel, F.D. & A. De Lange. 1983. Development of ovule and seed in Begonia squamulosa Hook.f. Acta botanica Neerlandica 32: 417-425.

Chauhan, R.S. 2016. Biotechnological approaches for conservation of rare, endangered and threatened plants. International Journal of Scientific and Research Publications 6(12): 10-14.

Espino, F.J., R. Linacero, J. Rueda, & A.M. Vazquez. 2004. Shoot regeneration in four Begonia genotypes. Biologia Plantarum 8(1): 101-104.

Flick, C.E., D.A. Evans, & W.R. Sharp. 1993. Organogenesis. in D.A. Evans, W.R. Sharp, P.V. Amirato, & T. Yamada (eds.). Handbook of Plant Cell Culture. Collier Macmillan Publisher. London. p.13-81.

Fonnesbech, M. 1974. The influence of NAA, BA and temperature on shoot and root development from Begonia x cheimantha petiole segments grown in vitro. Physiologia Plantarum 32: 49-54.

Gunawan. 1992. Teknik Kultur Jaringan. Pusat Antar Universitas Bioteknologi. Institut Pertanian Bogor. Bogor.

Haba, S.R. 2015. Conservation of Begonia Germplasm through Seeds: Characterization of Germination and Vigor in Different Species. Thesis. Master of Science in the Graduate School of The Ohio State University. Ohio.

Hoesen, D.S.H., 1998. Kultur jaringan kunir putih (Kaempferia rotunda L.). Berita Biologi. 4(4): 175-181.

Hughes, M. 2008. An Annotated Checklist of Southeast Asian Begonia. Royal Botanic Gardens. Edinburgh.

Kumari, S., P. Baskaran, & J. van Standen. 2017. In vitro regeneration of Begonia homonyma – a threatened plant. South African Journal of Botany 109: 174–177.

Kumaria, S., M. Kehie, S. Shekhar Das Bhowmik, M. Singh, & P. Tandon. 2012. In vitro regeneration of Begonia rubrovenia var. meisneri C.B. Clarke: A rare and endemic ornamental plant of Meghalaya, India. Indian Journal of Biotechnology. 11: 300-303.

Lee, D.J. 2002. The regulation of Korean radish cationic peroxidase promoter by a low ratio of cytokinin to auxin. Plant Science 162: 345–353.

Magdalita, P.M., D.G. Ian, A.D. Roderick, & W.A. Stephen. 1997. Effect of ethylene and culture environment on development of papaya nodal cultures. Plant Cell Tissue and Organ Culture 49: 93-100.

Mariska, I., E.G. Lestari, & D. Sukmadjaja. 1987. Multiplikasi tunas tanaman Mentha melalui kultur in vitro. Pemb. Littri 12(3-4): 80-84.

Mcdonald, M.B. 2005. flower seed Physiology and Plug Germination. In M.B. McDonald & F.Y. Kwong. (Eds.). Flower Seeds: Biology Technology. CABI Publishing. Cambridge. p.145-162.

Mendi, Y.Y., P. Curuk, E. Kocaman, C. Unek, S. Eldogan, G. Gencel, & S. Cetiner. 2009. Regeneration of Begonia plantlets by direct organogenesis. African Journal of Biotechnology 8(9): 1860-1863.

Murashige, T. & F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum 15: 473–497.

Nisha, M.C., S. Rajeshkumar, T. Selvaraj, & M.S. Subramanian. 2009. A valued Indian medicinal plant – Begonia malabarica Lam.: successful plant regeneration through various explants and field performance. Maejo International Journal.of Sciences and Technology 3(2): 261-268.

Peck, D.E. & B.G. Cumming. 1984. In vitro propagation of Begonia x tuberhybrida from leaf sections. HortScience 19(3): 395-397.

Purnamaningsih, R., I. Mariska, & S. Rahayu. 1988. Penekanan masalah penguningan pada daun Pulai. Plasma Nutfah 3(1): 1-7.

Reuter G. & N.N. Bhandari. 1981. Organogenesis and histogenesis of adventitious organs induced on leaf blade segments of Begonia elatior hybrids (Begonia×hiemalis) in tissue culture. Gartenbauwissenschaft 46: 241–249.

Rutty, R. (TPTNC). 2000. Begonia salaziensis. The IUCN Red List of Threatened Species 2000: e.T39460A10239643. Downloaded on 06 August 2017.

Salisbury, F.B. & C.W. Ross. 1992. Plant Physiology, Hormones and Plant Regulators: Auxins and Gibberellins. 4th Edition, Wadsworth Publishing, Belmont. p.357-381.

Siregar, H.M. 2017. The conservation of native, lowland Indonesian Begonia species (Begoniaceae) in Bogor Botanic Gardens. Biodiversitas 18(1): 326-333.

Takayama, S. & M. Misawa. 1981. Mass propagation of Begonia×hiemalis plantlets by shake culture. Plant Cell Physiology 22: 461–467.

Takayama S. & M. Misawa. 1982. Factors affecting differentiation in vitro and a mass-propagation scheme for Begonia× hiemalis. Scentiai Horticulturae 6: 65–75.

Tebbitt, M.C. 2005. Begonias: Cultivation, Identification, and Natural History. Brooklyn Botanical Garden. New York.

Tisserat, B., T. Murashige, & E.B. Esan. 1979. Somatic embryogenesis in Angiosperms. In. J. Janick (ed.). Horticultural Reviews vol 1. AVI Publishing Co. Inc. Wesport.

Watson, L. & M.J. Dallwitz. 1992. The Families of Flowering Plants: Descriptions, Illustration, Identification and Information Retrieval. Accessed 18 May 2012.

Welander, T. 1977. In vitro organogenesis in explants from different cultivars of Begonia x hiemalis. Physiologia Plantarum 41: 142-145.

West, M.M. & N.A. Lott. 1991. A histological and elemental analysis study of the mature seed of Begonia semperflorens. Canadian Journal of Botany 69: 2165-2169.

Yousef, G., G. Nematzadeh, R. Asgharzadeh, K.B. Shahin, & M. Yazdani. 2012. Effect of explants type and growth regulators on in vitro micropropagation of Begonia rex. International Research Journal of Applied and Basic Sciences 3(4): 896-901.

Zaer & Mapes. 1982. Action of growth regeneration. In. Bonga & Durzan (eds.) Tissue Culture in Forestry. Martinus Nijhoff. London. p.231-235.


Copyright (c) 2018 Buletin Kebun Raya

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Jurnal Buletin Kebun Raya

Diterbitkan oleh (published by):
Pusat Konservasi Tumbuhan Kebun Raya (Center For Plant Conservation Botanic Gardens)
Lembaga Ilmu Pengetahuan Indonesia (The Indonesian Institute of Sciences)
Alamat (address) : Pusat Konservasi Tumbuhan Kebun Raya-LIPI Jl. Ir. H. Juanda 13, Bogor 16003
Telepon (Phone) : (0251) 8322187
Email :
Website :

pISSN: 0125-961X

eISSN: 2460-1519