STUDI KOMPARASI KARAKTER MORFOLOGI DAN ISOZIM DUKU Lansium parasiticum (Osbeck) K.C. Sahni & Bennet ASAL KABUPATEN KUANTAN SINGINGI, RIAU

Ade Damayanti, Fitmawati Fitmawati, Herman Herman Viewed 89 times, Downloaded 251 times

Abstract


Duku (Lansium parasiticum (Osbeck) K.C. Sahni & Bennet) from Kuantan Singingi Regency, Riau, known by local people as duku turak, has a unique oval betel nut-like neck, and tastes sweet similar to round duku. It has thick skin that is not easy to break, nor quickly rot. This duku was more preferred by local people than the round shaped duku known as duku gondok. However, the information about the potential of duku turak has not been known widely. The aim of this study was to compare morphology and isozyme characteristics of duku turak and gondok growing in Kuantan Singingi Regency based on morphological and isozyme characteristics. The result showed that turak and gondok can be distinguished based on morphological characteristics i.e tree, leave and fruit shape. Analysis of isozyme banding patterns used esterase isozyme which shows variation in the resulting patterns formed two bands. Acid phospatase (ACP) isozyme formed eight bands and aspartate aminotransferase (AAT) isozyme formed only one band. However, peroksidase isozyme showed no different variation in the resulting patterns. Similarity coefficient value based on morphology characters was about 0.15 – 0.74, while value of isozyme analysis was about 0.29 – 1.00 as well as the combination among morphology characters and isozyme were about 0.24 – 0.71. The results of this study provide characteristic information and taxonomic groupings that can be useful as a type improvement program that relies heavily on available genetic diversity

Keywords


duku gondok, duku turak, isozyme, Kuantan Singingi, Lansium parasiticum, morphology

Full Text:

PDF

References


Amer, S.A.M. 2011. Genetic variability of the Saudi Arabian Uromastyx aegyptia microlepis using protein and isoenzymes electrophoreses. Advances in Bioscienceand Biotechnology 2: 27-32.

Aminah, S. 2003. Dampak Perubahan Agroklimat Pada Kualitas, Kuantitas dan Budidaya Duku (Lansium domesticum Corr.) Komering. Thesis, Program Studi Ilmu Lingkungan, Program Pascasarjana Universitas Indonesia, Jakarta.

Cahyarini, R.D., A. Yunus & E. Purwanto. 2004. Identifikasi Keragaman genetik beberapa varietas lokal kedelai di Jawa berdasarkan analisis isozim. Agrosains 6 (2): 79-83.

El-Beltagi, H.S., A.A. Mohamed & B.E. Ekki. 2011. Differences in some constituents, enzymes activity and electrophoretic characterization of different Rapeseed (Brassica napus L.) cultivars. Analele Universităţii din Oradea - Fascicula Biologie 18(1): 45-52.

Fitmawati, R. Fauziah, I. Hayati, N. Sofiyanti, E. Enou & Matra. 2017. Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer. Biodiversitas 18 (3): 1035-1040.

Hadiati, S. & D. Sukmadjaja. 2002. Keragaman pola pita beberapa varietas nenas berdasarkan analisis isozim. Jurnal Bioteknologi Pertanian 7 (20): 62-70.

Hailu, H.W., D.H. Kristiyanto, A.R.A. Altawi & S.M. Raqib. 2014. Isozyme electrophoresis and morphometric comparison of reed (Imperata cylindrica) adaptation to different altitudes.

International Journal of Innovation Research in Science, Engineering and Technology 3(5): 12389-12394.

Johnson, M., N. Janakiraman, K. Chalini, M. Narayani & V. Kalaiarasi. 2012. Studies on developmental variation of isoperoxidase and protein profile of Zea mays L. Journal of Stress Physiology and Biochemistry 8:16–23.

Kaswan, V., A. Joshi, S.R. Maloo. 2012. Assessment of isozyme diversity in the medicinal plant isabgol (Plantago ovata Forsk). Journal of Cell Tissue Research 12: 33-49.

Kumar, P., V.K. Gupta, A.K. Misra, D.R. Modi & B.K. Pandey. 2009. Potential of molecular markers in plant biotechnology. Journal of Plant Omics 2(4): 141-162.

Na’iem, M. 2000. Analisis isozim dan pemanfaatannya di bidang kehutanan. Yogyakarta.

Nasution, M.A. 2008. Analisis parameter genetik dan pengembangan kriteria seleksi bagi pemuliaan nenas (Ananas comosus (L.) Merr.) di Indonesia. Thesis, Program Pasca Sarjana, Institut Pertanian Bogor.

Pathak, K., D. Hatwal, R. Bist, D.C. Pathak & A.K. Gaur. 2011. Study of biochemical variability in the populations of Aconitum balfourii by soluble protein and isoenzyme electrophoretic patterns. Journal of Chemical and Pharmaceutical Research 3(3): 295-301.

Rolf, R.I.M. 1998. NTSYS-pc. Numerical taxonomy and multivariate analysis system. version 2.0. Department of Ecology and Evolution State of New York.

Rouf, A. 2007. Studi keragaman genetik tanaman jarak pagar (Jatropha curcas L.) di Jawa Tengah. Thesis, Program Pasca Sarjana, Universitas Negeri Sebelas Maret, Surakarta.

Sathisha, C.S.R., S. Prasad, R. Gowda & V. Anitalakshmi. 2012. Characterization of cultivars based on electrophoretic analysis of seed proteins and isozymes in sunflower (Helianthus annuus L.). Indian Journal of Plant Sciences 1(1): 35-38.

Sumarsono, T.B. Suparjana & E.S. Purwati. 2012. karakteristik morfologi dan pola pita isozim varietas mangga (Mangifera sp.) di Kabupaten Banyumas. Prosiding Seminar Nasional Pengembangan Sumber Daya Pedesaan dan Kearifan Lokal Berkelanjutan II. Purwokerto.

Wendel, J. F. & N. F. Weeden. 1989. Visualization and interpretation of plant isozyme. In: Soltis, D.E. & P.S. Soltis (Eds.) Isozymes in Plant Biology. Dioscorides Press. Portland, Oregon.

Widyastuti, Y.E. & F.B. Paimin. 1993. Mengenal Buah Unggul Indonesia. Penebar Swadaya. Jakarta.




DOI: http://dx.doi.org/10.14203/bkr.v20i2.415

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Buletin Kebun Raya

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Indexing Partners


    agora-journals.fao.org INTERNATIONAL INSTITUTE OF ORGANIZED RESEARCH (I2OR)ROAD DIRECTORYPKP INDEX
 
Jurnal Buletin Kebun Raya

Diterbitkan oleh (published by):
Pusat Konservasi Tumbuhan Kebun Raya (Center For Plant Conservation Botanic Gardens)
Lembaga Ilmu Pengetahuan Indonesia (The Indonesian Institute of Sciences)
Alamat (address) : Pusat Konservasi Tumbuhan Kebun Raya-LIPI Jl. Ir. H. Juanda 13, Bogor 16003
Telepon (Phone) : (0251) 8322187
Email : buletin.pktkr.lipi@gmail.com
Website : http://krbogor.lipi.go.id

pISSN: 0125-961X

eISSN: 2460-1519