INDUKSI POLIPLOIDI MENGGUNAKAN KOLKISIN SECARA IN VIVO PADA BIBIT ANGGREK BULAN (Phalaenopsis amabilis (L.) BLUME)

Eka Martha Della Rahayu, Dewi Sukma, M. Syukur, Sandra A. Aziz, Irawati Irawati Viewed 2041 times, Downloaded 3306 times

Abstract


Induksi poliploidi pada bibit Phalaenopsis amabilis telah dilakukan menggunakan kolkisin secara in vivo. Induksi poliploidi dilakukan dengan meneteskan kolkisin pada pucuk bibit P. amabilis.  Tujuan penelitian ini adalah untuk mendapatkan konsentrasi kolkisin yang efektif untuk induksi poliploidi bibit P. amabilis dan  menghasilkan bibit P. amabilis poliploid. Percobaan disusun dalam rancangan kelompok lengkap teracak dengan satu faktor, yaitu konsentrasi kolkisin. Pucuk bibit P. amabilis ditetesi 0,01 ml kolkisin (0, 1000, 2000, 3000, 4000, dan 5000 mg L-1). Hasil percobaan menunjukkan bahwa peningkatan konsentrasi kolkisin dari 1000 sampai 5000 mg L-1 tidak berpengaruh nyata terhadap persentase hidup dan pertumbuhan bibit pada 24 minggu setelah perlakuan (24 MSP). Bibit P. amabilis poliploid dapat dihasilkan pada penetesan kolkisin 1000, 3000, 4000, dan 5000 mg L-1 dengan konsentrasi kolkisin paling efektif adalah 5000 mg L-1. Bibit poliploid memiliki ukuran stomata lebih besar dari bibit diploid sebaliknya kerapatan stomatanya lebih rendah.


Keywords


kolkisin; kromosom; Phalaenopsis amabilis; poliploid; stomata

References


Ajalin I, F. Kobza, and J. Doležel. 2002. Ploidy identification of doubled chromosome number plants in Viola x wittrockiana Gams. M-1 generation. Horticultural Science (Prague) 29(1): 35–40.

Chang, W.C. 2007. In vitro morphogenesis and micro-propagation of orchids. In: Chen, W.H. and H.H. Chen (eds). Orchid Biotechnology. World Scientific, New Jersey: 45–64.

Chen, L., Y. Wang, & M. Zhao. 2006. In vitro induction and characterization of tetraploid Lychnis senno Siebold et Zucc. Horticultural Science 41(3):759–761.

Chen, W.H., C.Y. Tang, and Y.L. Kao. 2009. Ploidy doubling by in vitro culture of excised protocorms or protocorm–like bodies in Phalaenopsis species. Plant Cell, Tissue and Organ Culture 98 (2):229–238. 10.1007/s11240-009-9557-3Logo_TM.png

Manton, I. 1950. Problems of cytology and evolution in the Pteridophyta. Cambridge. The University Press. 10.1126/science.113.2940.533-aLogo_TM.png

Dhooghe, E, K. van Laere, T. Eeckhaut, L. Leus, and J. van Huylenbroeck. 2011. Mitotic chromosome doubling of plant tissues in vitro. Plant Cell, Tissue and Organ Culture 104 (3):359–373. 10.1007/s11240-010-9786-5Logo_TM.png

Djafarer, R. 2008. Phalaenopsis spesies: Jenis dan potensi untuk silangan. Penebar Swadaya, Depok.

Gantait, S., N. Mandal, S. Bhattacharyya, and P. K. Das. 2011. Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella. Plant Cell, Tissue and Organ Culture 106: 485–493. 10.1007/s11240-011-9947-1Logo_TM.png

Griesbach, R.J. 1985. Polyploidy in Phalaenopsis orchid improvement. The Journal of Heredity 76: 74–75.

Grouh, M.S.H., H. Meftahizade, N. Lotfi, V. Rahimi, and B. Baniasadi. 2011. Doubling the chromosome number of Salvia hains using colchicine: Evaluation of morphological traits of recovered plants. Journal of Medicinal Plants Research 5(19): 4892–4898.

Jadrná, P., O. Plavcová, and F. Kobza. 2010. Morphological changes in colchicine–treated Pelargonium × hortorum L.H. Bailey greenhouse plants. Horticultural Science (Prague) 37 (1): 27–33.

Kao, Y.Y., C.C. Lin, C.H. Huang, and Y.H. 2007. The cytogenetics of Phalaenopsis orchids. In: Chen, W.H. & H.H. Chen (eds). Orchid Biotechnology. World Scientific, New Jersey: 115–128.

Liu, G., Z. Li, and M. Bao. 2007. Colchicine–induced chromosome doubling in Platanus acerifolia and its effect on plant morphology. Euphytica 157 (1):145–154. 10.1007/s10681-007-9406-6Logo_TM.png

Miguel, T.P. and K.W. Leonhardt. 2011. In vitro polyploid induction of orchids using oryzalin. Scientia Horticulturae 130 (1): 314–319. 10.1016/j.scienta.2011.07.002Logo_TM.png

Omidbaigi, R., M. Mirzaee, M.E. Hassani, and M.S. Moghadam. 2010a. Induction and identification of polyploidy in basil (Ocimum basilicum L.) medicinal plant by colchicine treatment. International Journal of Plant Production 4(2): 87–98.

Omidbaigi, R., S. Yavari, M.E. Hassani, and S. Yavari. 2010b. Induction of autotetraploidy in dragonhead (Dracocephalum moldavica L.) by colchicine treatment. Journal of Fruit and Ornamental Plant Research 18(1): 23–35.

Sarathum, S., M. Hegele, S. Tantiviwat, and M. Nanakorn. 2010. Effect of concentration and duration of colchicine treatment on polyploidy induction in Dendrobium scabrilingue L. European Journal of Horticultural Science 75:123–127.

Sastrosumarjo, S. dan M. Syukur. 2013. Perilaku kromosom. Di dalam: Syukur, M. dan S. Sastrosumarjo (eds). Sitogenetika Tanaman. IPB Press, Bogor: 65–87.

Song, C., Liu S.J., Xiao, J., He, W.G., Zhou, Y., Qin, Q.B., Zhang, C., Liu, Y. 2012. Polyploid organisms. Science China Life Sciences 55 (4): 301–311. 10.1007/s11427-012-4310-2Logo_TM.png

Sulistianingsih, R., Z.A. Suyanto, dan A.E. Noer. 2004. Peningkatan kualitas anggrek Dendrobium hibrida dengan pemberian kolkhisin. Ilmu Pertanian 11 (1): 13–21.

Sweet, H.R. 1980. The genus Phalaenopsis. Day Printing Corp., Pomona, California. 124 hlm.

Tanaka, R. and H. Kamemoto. 1984. Chromosome in orchids: Counting and numbers. In: Arditti, J., (eds). Orchid Biology: Reviews and perspectives, III. Cornell University Press, London: 324–397.

Tang, C.Y., and W.H. Chen. 2007. Breeding and development of new varieties in Phalaenopsis. In: Chen, W.H. & H.H. Chen (Eds.). Orchid Biotechnology. World Scientific, New Jersey: 1–22.

Ye, Y.M., J. Tong, X.P. Shi, W. Yuan, and G.R. Li. 2010. Morphological and cytological studies of diploid and colchicine-induced tetraploid lines of crape myrtle (Lagerstroemia indica L.). Scientia Horticulturae 124 (1): 95–101. 10.1016/j.scienta.2009.12.016Logo_TM.png

Zeng, S.H., C.W. Chen, L. Hong, J.H. Liu, and X.X. Deng. 2006. In vitro induction, regeneration and analysis of autotetraploids derived from protoplasts and callus treated with colchicine in Citrus. Plant Cell, Tissue and Organ Culture 87:85–93. 10.1007/s11240-006-9142-yLogo_TM.png




DOI: http://dx.doi.org/10.14203/bkr.v18i1.156

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Buletin Kebun Raya (The Botanic Gardens Bulletin)

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Indexing Partners


    agora-journals.fao.org INTERNATIONAL INSTITUTE OF ORGANIZED RESEARCH (I2OR)ROAD DIRECTORYPKP INDEX
 
Jurnal Buletin Kebun Raya

Diterbitkan oleh (published by):
Pusat Konservasi Tumbuhan Kebun Raya (Center For Plant Conservation Botanic Gardens)
Lembaga Ilmu Pengetahuan Indonesia (The Indonesian Institute of Sciences)
Alamat (address) : Pusat Konservasi Tumbuhan Kebun Raya-LIPI Jl. Ir. H. Juanda 13, Bogor 16003
Telepon (Phone) : (0251) 8322187
Email : buletin.pktkr.lipi@gmail.com
Website : http://krbogor.lipi.go.id

pISSN: 0125-961X

eISSN: 2460-1519