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Ekinase (Echinacea purpurea (L.) Moench) a medicinal plant that has immunostimulatory activity. This plant has been cultivating in Tawangmangu region by Medicinal Plant and Traditional Medicine Research and Development Office since 2002. Ten accessions of E. purpurea were found based on their morphological variation, three accessions of them are selected as promising accessions namely BH2, BHU3 dan BHU5. The objective of this research was to observe the genetic diversity of  those  promising accessions and 8 variants accession from mass selection year I using RAPD analysis. Those accessions were amplified using 10 RAPD primers. A total of 64 scorable fragments were generated from 9 RAPD primers, among which 48 fragments (75%) were polymorphic. The Dice coefficient was used to calculated the genetic similarity and UPGMA was used to generate the dendogram. The genetic similarity index among accessions evaluated ranged from 75,49-84,21% thus indicating that low level of genetic diversity. RAPD analysis proved to be efficient for genetic diversity of ekinase accessions from mass selection year I.


Ekinase (Echinacea purpurea (L.) Moench); genetic diversity; mass selection year I


Abdelmigid, HM. 2012. Efficiency of random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers for genotype fingerprinting and genetic diversity studies in canola (Brassica napus). African Journal of Biotechnology 11(24): 6409-6419.

Amri, E. and F. Mamboya. 2012. Genetic diversity in Pterocarpus angolensis populations detected by random amplified polymorphic DNA markers. International Journal of Plant Breeding and Genetics 6(2): 105-114. 10.3923/ijpbg.2012.105.114Logo_TM.png

Asolkar, T., AR. Desai and NP. Singh. 2011. Molecular analysis of cashew genotypes and their half-sib progeny using RAPD marker. Journal of Agricultural Technology 7(4):1097-1106.

Baig, MNR., S. Grewal and S. Dhillon. 2009. Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers. Turkish Journal of Agriculture and Forestry 33: 375-384. 10.3906/tar-0804-27Logo_TM.png

Baum, BR., SE. Binns, S. Mechanda, JT. Arnason, 1999. The Echinacea germ plasm enhancement project. in: Proceedings of the 1999 International Echinacea Symposium, Jun 3-5, Kansas City, MO.

Bishnoi, UR., J.E. Willisand S.R. Mentreddy. 2010. Methods to improve seed germination of purple coneflower (Echinacea purpurea (L.) Moench). Agriculture and Biology Journal of North America 1(3):185-188.

Chen, CL., SC. Zhang and JM. Sung. 2008. Biomass and caffeoyl phenols production of Echinacea purpurea grown in Taiwan. Experimental Agricuture 44(4): 497-507. 10.1017/S0014479708006753Logo_TM.png

Faleiro, FG., ACQ. Pinto, MCR. Cordeiro, SRM. Andrade, VHV. Ramos, G. Belon and JN. Dias. 2009. Genetic variability of mango cultivars developed by Embrapa Cerrados breeding program using RAPD markers. Acta Horticulturae 820: 177-182. 10.17660/ActaHortic.2009.820.18Logo_TM.png

Fateh E., N. Malekpourlashkariani and F. Gerami, 2012. Effects of various treatments on Echinacea purpurea L. seed dormancy breaking and germination. International Journal of Plant, Animal and Environmental Sciences 2(3):188-194.

Fu, XP., GG. Ning, LP. Gao and MZ. Bao. 2008. Genetic diversity of Dianthus accessions as assessed using two molecular marker systems (SRAPs and ISSRs) and morphological traits. Scientia Horticulturae 117(3): 263-270. 10.1016/j.scienta.2008.04.001Logo_TM.png

Hasan, SMZ., M. Shafie, B. Shafieand RM. 'Shah. 2009. Analysis of random amplified polymorphic DNA (RAPD) of Artemisia capillaries (wormwood capillary) in East Coast of Peninsular Malaysia. World Applied Sciences Journal 6(7): 976-986.

Hasnaoui, N., M. Messaound, J. Chibaniand, M. Trifi. 2010. Molecular polymorphisms in Tunisian pomegranate (Punica granatum L.) as revealed by RAPD fingerprints. Diversity 2(1): 107-114. 10.3390/d2010107Logo_TM.png

Kusumadewi, Y., YS. Purba dan T. Partomihardjo. 2010. Keragaman genetika ramin [Gonystylus bancanus (Miq.) Kurz] dari Provinsi Riau berdasarkan profil random amplified polymorphic DNA. Jurnal Biologi Indonesia 6(2): 173-183.

Lee, TT., CL. Chen, Z.H. Shieh, JC. Lin and B. Yu. 2009. Study on antioxidant activity of Echinacea purpurea L. extracts and its impact on cell viability. African Journal of Biotechnology 8(19): 5097-5105.

Matthias, A., L. Banbury, KM. Bone, DN. Leach and R.P. Lehmann. 2008. Echinacea alkylamides modulate induced immune responses in Tcells. Fitoterapia 79(1): 53-58. 10.1016/j.fitote.2007.07.012Logo_TM.png

Meng, L., HX. Yang, PC. Mao, HW. Gao and F.D. Sun. 2011. Genetic diversity analysis of Arrhenatherum elatius germplasm with inter-simple sequence repeat (ISSR) markers. African Journal of Biotechnology 10(44): 8729-8736.

Noormohammadi Z., FS. Jesvaghani, M. Sheida, F. Farahani and 0. Alishah, 2011. Inter simple sequence repeats (ISSR) and random amplified polymorphic DNA (RAPD) analyses of genetic diversity in Mehr cotton cultivar and its crossing progenies. African Journal of Biotechnology 10(56): 11839-11847.

Poerba, YS. dan D. Martanti. 2008. Keragaman genetik berdasarkan marka random

amplified polymorphic DNA pada Amorphophallus muelleri Blume di Jawa. Biodiversitas 9(4): 245-249. 10.13057/biodiv/d09040Logo_TM.png

Resmi, J. and I. Sreelathakumary. 2011. RAPD markers for genetic variability studies in ashgourd Benincasa hispida (Thunb.) Cogn. Journal of Agricultural Technology 7(4): 1097-1106.

Rohlf, FJ. 1998. NTSYS-pc Numerical Taxonomy andMultivariate Analysis System,Version 2. 00. Exeter Software, Setauket, New York.

Rout, ZN. 2006. Identification of Tinospora cordifolia (Willd.) Miers ex Hook.f. & Thomas Using RAPD Markers. Zeitschrift fur Naturforschung Section C, Biosciences 61(1/2): 118-122.

Runtunuwu, DS., JEX. Rogi dan JH. Palendeng. 2011. Identifikasi varietas kentang "SuperJohn" berdasarkan penanda RAPD (random amplified polymorphic DNA). Eugenia 17(1):52-59.

Subositi, D. and Fauzi. 2011. Morphological variation of Echinacea purpurea (L.) Moench accessions in Medicinal Plant and Traditional Medicine Research and Development Office. Proceedings of The 2nd International Symposium on Temulawak. The 40th meeting National Working Group on Indonesian Medicinal Plants. Biopharmaca Research Center.

Susantidiana, A. Wijaya, B. Lakitan dan M. Surahman. 2009. Identifikasi beberapa aksesi jarak pagar (Jatropha curcas L.) melalui analisis RAPD dan morfologi. Jurnal Agronomi Indonesia 37(2): 167- 173.

Thygesen, L., J. Thulin, A. Mortensen, LH. Skibsted and P. Molgaard. 2007. Antioxidant activity of cichoric acid and alkamides from Echinacea purpurea, alone and in combination. Food Chemistry 101(1): 74-81. 10.1016/j.foodchem.2005.11.048Logo_TM.png

Zappacosta, D., M. Meier, A. Carrera, G. Pacheco, S. Cardone, JP. Silva and V. Echenique. 2011. Molecular markers to study the variability within the Eragrostis curvula complex. International Journal of Experimental Botany 80(2): 211-220.



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